RBM25 antibody (AA 100-820)

Catalog No. ABIN7599620

The Rabbit Polyclonal anti-RBM25 antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect RBM25 in samples from Mouse, Human and Rat.

Quick Overview for RBM25 antibody (AA 100-820) (ABIN7599620)

Target: RBM25 (RNA Binding Motif Protein 25 (RBM25))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-RBM25 Antibody

Binding Specificity: AA 100-820

Purpose: Anti-RBM25 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RBM25 Antibody Picoband® (ABIN7599620). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RBM25 recombinant protein (Position: D100-E820).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fortes, P., Longman, D., McCracken, S., Ip, J. Y., Poot, R., Mattaj, I. W., Caceres, J. F., Blencowe, B. J. Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3-prime-end formation. FEBS Lett. 581: 3087-3097, 2007. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 11/20/2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RBM25

Target: RBM25 (RNA Binding Motif Protein 25 (RBM25))

Alternative Name: RBM25

Background:

Synonyms: Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, Ppase, PPA1, IOPPP, PP

Tissue Specificity: Expressed ubiquitously.

Background: RNA-binding protein 25 is a protein that in humans is encoded by the RBM25 gene. Enables mRNA binding activity. Involved in regulation of alternative mRNA splicing, via spliceosome and regulation of apoptotic process. Located in nuclear speck.

Molecular Weight: 110-120 kDa

Gene ID: 58517

UniProt: P49756